Interaction between Lipid Monolayers and Poloxamer 188: An X-Ray Reflectivity and Diffraction Study
| Autor: | Guohui Wu, Markus J. Weygand, Canay Ege, Kristian Kjaer, Ka Yee C. Lee, Jaroslaw Majewski |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
1 2-Dipalmitoylphosphatidylcholine Surface Properties Membrane lipids Lipid Bilayers Biophysics Electrons 02 engineering and technology Poloxamer 010402 general chemistry 01 natural sciences Membrane Lipids Surface-Active Agents X-Ray Diffraction Monolayer Membrane fluidity Pressure Scattering Radiation Lipid bilayer phase behavior Lipid bilayer Phospholipids Models Statistical Membranes Chemistry Bilayer X-Rays Cell Membrane Temperature Water Lipid metabolism Phosphatidylglycerols 021001 nanoscience & nanotechnology Lipid Metabolism Lipids 0104 chemical sciences X-ray reflectivity Crystallography Microscopy Fluorescence lipids (amino acids peptides and proteins) 0210 nano-technology |
| Zdroj: | Biophysical Journal. 89(5):3159-3173 |
| ISSN: | 0006-3495 |
| DOI: | 10.1529/biophysj.104.052290 |
| Popis: | The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored. |
| Databáze: | OpenAIRE |
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