The Permeability of Reconstituted Nuclear Pores Provides Direct Evidence for the Selective Phase Model
| Autor: | Bastian B. Hülsmann, Dirk Görlich, Aksana A. Labokha |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Xenopus
Molecular Sequence Data Active Transport Cell Nucleus Context (language use) Saccharomyces cerevisiae Xenopus Proteins Models Biological Permeability General Biochemistry Genetics and Molecular Biology Xenopus laevis medicine Animals Nuclear pore biology Biochemistry Genetics and Molecular Biology(all) biology.organism_classification Protein Structure Tertiary Nuclear Pore Complex Proteins medicine.anatomical_structure Permeability (electromagnetism) Cytoplasm Nuclear Pore Biophysics Nucleoporin Nuclear transport Nucleus |
| Zdroj: | Cell |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2012.07.019 |
| Popis: | SummaryNuclear pore complexes (NPCs) maintain a permeability barrier between the nucleus and the cytoplasm through FG-repeat-containing nucleoporins (Nups). We previously proposed a “selective phase model” in which the FG repeats interact with one another to form a sieve-like barrier that can be locally disrupted by the binding of nuclear transport receptors (NTRs), but not by inert macromolecules, allowing selective passage of NTRs and associated cargo. Here, we provide direct evidence for this model in a physiological context. By using NPCs reconstituted from Xenopus laevis egg extracts, we show that Nup98 is essential for maintaining the permeability barrier. Specifically, the multivalent cohesion between FG repeats is required, including cohesive FG repeats close to the anchorage point to the NPC scaffold. Our data exclude alternative models that are based solely on an interaction between the FG repeats and NTRs and indicate that the barrier is formed by a sieve-like FG hydrogel. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|