Robot‐assisted mechanical therapy attenuates stroke‐induced limb skeletal muscle injury
| Autor: | Surya Gnyawali, Savita Khanna, Seth Teplitsky, Mallory Heigel, Cameron Rink, Richard Stewart, Maria H.H. Balch, Chandan K. Sen, Hallie Harris |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.medical_treatment Ischemia Biochemistry 03 medical and health sciences 0302 clinical medicine Physical medicine and rehabilitation Genetics medicine Animals Rats Wistar Muscle Skeletal Molecular Biology Stroke Physical Therapy Modalities Rehabilitation business.industry Brain-Derived Neurotrophic Factor Research Stroke Rehabilitation Skeletal muscle Robotics Myostatin medicine.disease Hindlimb Rats 030104 developmental biology medicine.anatomical_structure Regional Blood Flow Preclinical testing Cytokines business 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | The FASEB Journal. 31:927-936 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | The efficacy and optimization of poststroke physical therapy paradigms is challenged in part by a lack of objective tools available to researchers for systematic preclinical testing. This work represents a maiden effort to develop a robot-assisted mechanical therapy (RAMT) device to objectively address the significance of mechanical physiotherapy on poststroke outcomes. Wistar rats were subjected to right hemisphere middle-cerebral artery occlusion and reperfusion. After 24 h, rats were split into control (RAMT−) or RAMT+ groups (30 min daily RAMT over the stroke-affected gastrocnemius) and were followed up to poststroke d 14. RAMT+ increased perfusion 1.5-fold in stroke-affected gastrocnemius as compared to RAMT− controls. Furthermore, RAMT+ rats demonstrated improved poststroke track width (11% wider), stride length (21% longer), and travel distance (61% greater), as objectively measured using software-automated testing platforms. Stroke injury acutely increased myostatin (3-fold) and lowered brain-derived neurotrophic factor (BDNF) expression (0.6-fold) in the stroke-affected gastrocnemius, as compared to the contralateral one. RAMT attenuated the stroke-induced increase in myostatin and increased BDNF expression in skeletal muscle. Additional RAMT-sensitive myokine targets in skeletal muscle (IL-1ra and IP-10/CXCL10) were identified from a cytokine array. Taken together, outcomes suggest stroke acutely influences signal transduction in hindlimb skeletal muscle. Regimens based on mechanical therapy have the clear potential to protect hindlimb function from such adverse influence.—Sen, C. K., Khanna, S., Harris, H., Stewart, R., Balch, M., Heigel, M., Teplitsky, S., Gnyawali, S., Rink, C. Robot-assisted mechanical therapy attenuates stroke-induced limb skeletal muscle injury. |
| Databáze: | OpenAIRE |
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