Expression of the gp150 maedi visna virus envelope precursor protein by mammalian expression vectors
| Autor: | Gordon D. Harkiss, Francesco Tolari, Lluís Luján, Cyril Barbezange, Maria Luisa Carrozza, Christophe Fraisier, Marie Suzan-Monti, Michel Pépin, Beatriz Amorena, H. Arnarson, Sergio Rosati, Damián F. de Andrés, Valgerdur Andrésdŏttir, Barbara Blacklaws |
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| Rok vydání: | 2007 |
| Předmět: |
Visna-maedi virus
Visna virus viruses Recombinant Fusion Proteins Genetic Vectors Transfection Genes env Virus DNA vaccination Cell Line Visna Plasmid Viral envelope Virology Vaccines DNA Animals Humans Vector (molecular biology) Protein Precursors Expression vector biology virus diseases Gene Products env Viral Vaccines biology.organism_classification Molecular biology Plasmids |
| Zdroj: | Journal of virological methods. 146(1-2) |
| ISSN: | 0166-0934 |
| Popis: | There are very few previous reports of expression of native full-length maedi visna virus (MVV) Env gp150 protein in the literature. Therefore the use of different plasmid and viral expression vectors to obtain full-length gp150 was investigated. A mammalian expression plasmid, pN3-Env, was constructed containing the MVV env gene encoding the precursor protein gp150 Env. The functionality of the recombinant plasmid was tested for expression in HEK293 cells. A recombinant modified vaccinia Ankara virus, MVA-Env, with expression detected in avian cells was also made. The expression of the MVV gp150 Env precursor protein was shown for the first time upon transfection of the eukaryotic HEK293 cells by the pN3-Env plasmid DNA as demonstrated by Western blot analysis. These plasmid or viral expression vectors are of potential use in MVV vaccines. |
| Databáze: | OpenAIRE |
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