Activation of ATP-sensitive potassium channels as an element of the neuroprotective effects of the Traditional Chinese Medicine MLC901 against oxygen glucose deprivation

Autor: Catherine Widmann, H. Moha Ou Maati, Marc Borsotto, F. Chatelain, M Lazdunski, Catherine Heurteaux
Přispěvatelé: Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Calcium imaging
Pharmacology
Membrane Potentials
Glibenclamide
Mice
0302 clinical medicine
KATP Channels
Ischemia
Chlorocebus aethiops
Hypoxia
Cells
Cultured
Cerebral Cortex
Neurons
0303 health sciences
KATP channel
Hyperpolarization (biology)
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
Potassium channel
Cell Hypoxia
3. Good health
Neuroprotective Agents
MLC901
COS Cells
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
medicine.drug
Cell Survival
Nerve Tissue Proteins
Carbohydrate metabolism
Neuroprotection
Cell Line
03 medical and health sciences
Cellular and Molecular Neuroscience
Membrane Transport Modulators
medicine
Potassium Channel Blockers
Animals
Calcium Signaling
030304 developmental biology
Activator (genetics)
business.industry
medicine.disease
Embryo
Mammalian
Rats
Mice
Inbred C57BL
Glucose
nervous system
business
030217 neurology & neurosurgery
Drugs
Chinese Herbal
Zdroj: Neuropharmacology
Neuropharmacology, Elsevier, 2012, 63 (4), pp.692-700. ⟨10.1016/j.neuropharm.2012.05.035⟩
ISSN: 0028-3908
Popis: International audience; NeuroAid (MLC601 and MLC901), a Traditional Medicine used in China for patients after stroke has been reported in preclinical models of ischemia to induce neuroprotection and neuroplasticity. This work shows the effects of MLC901 on an in vitro model of oxygen glucose deprivation (OGD). MLC901 prevents neuronal death induced by 120 min OGD and decreases the exaggerated Ca(2+) entry in mature cortical neurons exposed to 120 min OGD. The neuroprotective effect of MLC901 is associated with a large hyperpolarization of ∼20 mV which is antagonized by glibenclamide, the specific inhibitor of K(ATP) channels. In addition MLC901 strengthens the activation of K(ATP) channels. MLC901 has been directly shown to act as an activator of K(ATP) channels as potent as the classical K(ATP) channel opener. The capacity of MLC901 to produce a large hyperpolarization, particularly in neurons that have suffered from energy deprivation probably plays an important role in the neuroprotective effects of this traditional medicine that comes in addition to its previously demonstrated neuroregenerative properties.
Databáze: OpenAIRE
Externí odkaz: