Mechanophenotyping of B16 Melanoma Cell Variants for the Assessment of the Efficacy of (-)-Epigallocatechin Gallate Treatment Using a Tapered Microfluidic Device
| Autor: | Shukei Sugita, Daichi Ono, Masanori Nakamura |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
lcsh:Mechanical engineering and machinery
Microfluidics Cell microfluidics mechanophenotyping chemical and pharmacologic phenomena 02 engineering and technology Epigallocatechin gallate Article Metastasis Extracellular matrix 03 medical and health sciences chemistry.chemical_compound medicine cancer lcsh:TJ1-1570 Electrical and Electronic Engineering neoplasms 030304 developmental biology 0303 health sciences Mechanical Engineering Cancer hemic and immune systems 021001 nanoscience & nanotechnology medicine.disease respiratory tract diseases medicine.anatomical_structure chemistry Control and Systems Engineering metastatic potential Cancer cell Cancer research biological phenomena cell phenomena and immunity 0210 nano-technology B16 melanoma |
| Zdroj: | Micromachines Volume 10 Issue 3 Micromachines, Vol 10, Iss 3, p 207 (2019) |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi10030207 |
| Popis: | Metastatic cancer cells are known to have a smaller cell stiffness than healthy cells because the small stiffness is beneficial for passing through the extracellular matrix when the cancer cells instigate a metastatic process. Here we developed a simple and handy microfluidic system to assess metastatic capacity of the cancer cells from a mechanical point of view. A tapered microchannel was devised through which a cell was compressed while passing. Two metastasis B16 melanoma variants (B16-F1 and B16-F10) were examined. The shape recovery process of the cell from a compressed state was evaluated with the Kelvin&ndash Voigt model. The results demonstrated that the B16-F10 cells showed a larger time constant of shape recovery than B16-F1 cells, although no significant difference in the initial strain was observed between B16-F1 cells and B16-F10 cells. We further investigated effects of catechin on the cell deformability and found that the deformability of B16-F10 cells was significantly decreased and became equivalent to that of untreated B16-F1 cells. These results addressed the utility of the present system to handily but roughly assess the metastatic capacity of cancer cells and to investigate drug efficacy on the metastatic capacity. |
| Databáze: | OpenAIRE |
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