Krüppel-like factor 4 (gut-enriched Krüppel-like factor) inhibits cell proliferation by blocking G1/S progression of the cell cycle
| Autor: | Duyen T. Dang, Gina Hamlin, Xinming Chen, Eduardo Marbán, Vincent W. Yang, David C. Johns, Ronggai Sun, Deborah E. Geiman |
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| Rok vydání: | 2001 |
| Předmět: |
Ecdysone
Time Factors Cell division Blotting Western Kruppel-Like Transcription Factors CHO Cells Cell Separation Biology Transfection Biochemistry Article Adenoviridae Cell Line S Phase Kruppel-Like Factor 4 stomatognathic system Cricetinae Tumor Cells Cultured Animals Humans Promoter Regions Genetic Molecular Biology Transcription factor S phase Cell growth Chinese hamster ovary cell fungi Cell Cycle G1 Phase Cell Biology DNA Cell cycle Fibroblasts Blotting Northern Flow Cytometry Molecular biology Cell biology DNA-Binding Proteins Ecdysterone Cell culture KLF4 embryonic structures Drosophila sense organs Cell Division Plasmids Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 276(32) |
| ISSN: | 0021-9258 |
| Popis: | Kruppel-like factor 4 (KLF4) is an epithelial cell-enriched, zinc finger-containing transcription factor, the expression of which is associated with growth arrest. Previous studies show that constitutive expression of KLF4 inhibits DNA synthesis but the manner by which KLF4 exerts this effect is unclear. In the present study, we developed a system in which expression of KLF4 is controlled by a promoter that is induced upon treatment of cells containing the receptors for the insect hormone, ecdysone, with ponasterone A, an ecdysone analogue. The rate of proliferation of a stably transfected colon cancer cell line, RKO, was significantly decreased following addition of ponasterone A when compared with untreated cells. Flow cytometric analyses indicated that the inducible expression of KLF4 caused a block in the G(1)/S phase of the cell cycle. A similar block was observed when ecdysone receptor-containing RKO cells were infected with a replication-defective recombinant adenovirus containing an inducible KLF4 and treated with ponasterone A. Results of these studies provide evidence that the inhibitory effect of KLF4 on cell proliferation is mainly exerted at the G(1)/S boundary of the cell cycle. |
| Databáze: | OpenAIRE |
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