Expression pattern of the apoptosis-stimulating protein of p53 family in p53+ human breast cancer cell lines
| Autor: | Xue-xia Lv, Yanping Chen, Ping Wang, Chun-fang Gao, Changsong Wang, Jian Yin |
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| Rok vydání: | 2013 |
| Předmět: |
Tumor cell apoptosis
p53 Cancer Research ASPP family member business.industry Apoptosis Breast cancer cell line medicine.disease Bioinformatics Causes of cancer Breast cancer Oncology Expression pattern Cell culture Genetics medicine Cancer research Cancer cell lines business Primary Research Human breast |
| Zdroj: | Cancer Cell International |
| ISSN: | 1475-2867 |
| Popis: | Background The apoptosis-stimulating protein of p53 (ASPP) family comprises three members, namely, ASPP1, ASPP2, and iASPP. They regulate the promotive effect of p53 on apoptosis. Breast cancer (BC) remains as one of the leading causes of cancer or cancer-related mortality among women. However, the relationship between the ASPP family members and p53, as well as the dissemination and expression pattern of ASPP family members in p53+ BC, has not been elucidated. Our objectives are to detect the expression of ASPP family members in p53+ BC cell lines and determine its significance in tumor cell apoptosis. Methods The mRNA expression of ASPP family members in five p53+ BC cell lines was detected through RT-PCR and assayed using Quality-one software. The p53 protein expression was detected by immunohistochemistry. Afterward, the apoptosis indices of the five BC cell lines were detected by flow cytometry. Results The iASPP mRNA was expressed in Bcap-37, MCF-7, and HBL-100. Compared with the human peripheral blood mononuclear cells, significant differences were found in the ASPP1 mRNA in Bcap-37, MDA-MB-231, MCF-7, and HBL-100 (p 0.05). The ASPP2 mRNA was expressed in MDA-MB-231, Bcap-37, and MCF-7, but not in HBL-100 and ZR-75-30. The p53 protein was expressed in five breast cancer cell lines. ZR-75-30 and MDA-MB-231 apoptosis indices were higher than those of other breast cancer cell line and peripheral blood mononuclear cells (p |
| Databáze: | OpenAIRE |
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