Establishment of Primary Cell Culture from Hepatopancreas of Penaeus monodon for the Study of White Spot Syndrome Virus (WSSV)

Autor:	G. Ravikumar, Arumugam Uma, T.G. Prabhakar, A. Koteeswaran
Rok vydání:	2002
Předmět:	Lysis Ecology biology White spot syndrome SH1-691 Aquatic Science biology.organism_classification Virus Penaeus monodon Microbiology Streptomycin Cell culture Aquaculture. Fisheries. Angling medicine Hepatopancreas Fetal bovine serum Food Science medicine.drug
Zdroj:	Asian Fisheries Science, Vol 15, Iss 4 (2002)
ISSN:	2073-3720 0116-6514
DOI:	10.33997/j.afs.2002.15.4.008
Popis:	A primary cell culture system was developed from the cells of hepatopancrea s of Penaeus monodon. Minced tissues of hepatopancrea s were seeded (10 6 cells·cm 3 ) and incubated at 28 o C in single strength Leibovitz’s L-15 medium (pH 7.2) supplemented with 10% fetal bovine serum and antibiotics (ciprofloxicin 100 mg·ml; penicillin 100 IU·ml; streptomycin 100 mg·ml and nystatin 25 mg·ml). The cells showed good attachment to the culture vessels by 24h and formed a confluent monolayer within 72 h. The primary cell culture could be maintained up to a maximum period of 12 weeks by subculturing seven times. When whitespot syndrome virus (WSSV) was inoculated in the hepatopancreas cell culture, characteristic cytopathic effects which include rounding of cells, detachment, lysis of cell sheet with circular clear areas of depletion of cells were observed within 120 h.
Databáze:	OpenAIRE
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