'Fishing and Hunting'—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
| Autor: | Andrea Varga, Evelin Sánta-Bell, Diána Balogh-Weiser, Zsófia Molnár, Flóra Nagy, László Poppe, Gábor Hornyánszky, Csaba Paizs |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
magnetic nanoparticles
Pharmaceutical Science selective enzyme immobilization Phenylalanine Phenylalanine ammonia-lyase Article Analytical Chemistry law.invention lcsh:QD241-441 lcsh:Organic chemistry law Drug Discovery Chelation Physical and Theoretical Chemistry Plant Proteins chemistry.chemical_classification Organic Chemistry Enzymes Immobilized Combinatorial chemistry Recombinant Proteins Enzyme chemistry Chemistry (miscellaneous) Covalent bond phenylalanine ammonia-lyase Recombinant DNA Molecular Medicine Petroselinum Fermentation IMAC Linker |
| Zdroj: | Molecules Volume 24 Issue 22 Molecules, Vol 24, Iss 22, p 4146 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24224146 |
| Popis: | This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag. |
| Databáze: | OpenAIRE |
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