Alterations of gut microbiome and metabolite profiles in choledocholithiasis concurrent with cholangitis
| Autor: | Binbin Li, Pei-Mei Shi, Kaiming Wu, Yong Lin, Xin Zeng, Yuan-Yuan Luo, Yiting Lu, Zhiyuan Hao, Kegong Tao, Pei-Qin Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Cholangitis Metabolite Inflammation Gut flora digestive system Pathogenesis chemistry.chemical_compound Kynurenic acid Internal medicine medicine Humans Hepatology biology business.industry biology.organism_classification Sphingolipid Gastrointestinal Microbiome Choledocholithiasis chemistry Metagenomics Case-Control Studies Immunology medicine.symptom business |
| Zdroj: | Hepatology International. 16:447-462 |
| ISSN: | 1936-0541 1936-0533 |
| DOI: | 10.1007/s12072-021-10231-5 |
| Popis: | Gut microbiota and their metabolic products might play important roles in regulating the pathogenesis of choledocholithiasis concurrent with cholangitis (CC). The aim of this study was to explore the characteristic gut dysbiosis, metabolite profiles and the possible roles in patients with CC.A case-control study was carried out to analyze the alterations in the intestinal microbiota and their metabolites in patients with CC (n = 25) compared with healthy controls (HCs) (n = 25) by metagenomic sequencing to define the gut microbiota community and liquid chromatography/mass spectrometry (LC/MS) analysis to characterize the metabolite profiles.Significantly reduced Shannon diversity index (p = 0.043) and differential overall fecal microbiota community in CCs were observed. Twelve dominant altered species were identified and analyzed (LDA score 3.0, p 0.05) (Q value 0.05), including unclassified_f_Enterobacteriaceae, Escherichia_coli, Roseburia_faecis and Eubacterium rectale. Moreover, the levels of KEGG pathways related to biofilm formation of Escherichia coli, lipopolysaccharide (LPS) biosynthesis, and the metabolism of propanoate and glutathione in CCs were significantly altered. Finally, 47 markedly changed metabolites (VIP 1.0 and p 0.05), including low level of kynurenic acid (KYNA) and high concentration of N-palmitoylsphingosine involving tryptophan metabolism and sphingolipid signaling pathways, were identified to validate aberrant metabolic patterns in CCs, and multiple correlated metabolic modules involving bile inflammation were altered in CCs.Our study provides novel insights into compositional and functional alterations in the gut microbiome and metabolite profiles in CC and the underlying mechanisms between gut microbiota and bile inflammation. |
| Databáze: | OpenAIRE |
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