Immunoprofiling reveals novel mast cell receptors and the continuous nature of human lung mast cell heterogeneity
| Autor: | Jesper Säfholm, Mikael Adner, Mamdoh Al-Ameri, Elin Rönnberg, Avinash Ravindran, Daryl Boey Zhong Hao, Joakim S. Dahlin, Ann-Charlotte Orre, Gunnar Nilsson, Sven-Erik Dahlén |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
FcεRI
Cell Plasticity Immunology Population carboxypeptidase A3 (CPA3) CD34 Gene Expression Receptors Cell Surface Biology heterogenity Immunophenotyping Flow cytometry CD49a SUSD2 Fc epsilon RI medicine Humans Mast Cells education Receptor Lung Original Research education.field_of_study medicine.diagnostic_test Receptors IgE Immunology in the medical area Cell Differentiation human lung mast cells Flow Cytometry Mast cell Immunohistochemistry Cell biology Gene expression profiling medicine.anatomical_structure Immunologi inom det medicinska området Immunologi chymase (CMA1) Biomarkers CD38 Peptide Hydrolases |
| Zdroj: | Frontiers in Immunology |
| DOI: | 10.22541/au.161696545.56545800/v1 |
| Popis: | Background: Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population of cells, with distinct subpopulations identified. However, a systematic and comprehensive analysis of cell-surface markers to study human lung mast cell heterogeneity has yet to be performed. Methods: Human lung mast cells were obtained from lung lobectomies, and the expression of 332 cell-surface markers was analyzed using flow cytometry and the LEGENDScreen kit. Markers that exhibited high variance were selected for additional analyses to reveal whether they were correlated and whether discrete mast cell subpopulations were discernable. Results: We identified the expression of 102 surface markers on human lung mast cells. Several markers showed high continuous variation in expression within the mast cell population. Six of these markers were correlated: SUSD2, CD49a, CD326, CD34, CD66 and HLA-DR. The expression of these markers was also correlated with the size and granularity of mast cells. However, no marker produced an expression profile consistent with a bi- or multimodal distribution. Conclusions: LEGENDScreen analysis identified more than 100 cell-surface markers on mast cells, including 23 that, to the best of our knowledge, have not been previously described on human mast cells. Several of the newly described markers are known to be involved in sensing the microenvironment, and their identification can shed new light on mast cell functions. The exhaustive expression profiling of the 332 surface markers failed to detect distinct mast cell subpopulations. Instead, we demonstrate the continuous nature of human lung mast cell heterogeneity. |
| Databáze: | OpenAIRE |
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