Effect of Overfeeding Shetland Pony Mares on Embryonic Glucose and Lipid Accumulation, and Expression of Imprinted Genes
| Autor: | D' Fonseca, Nicky M M, Gibson, Charlotte M E, van Doorn, David A, Roelfsema, Ellen, de Ruijter-Villani, Marta, Stout, Tom A E, Interne geneeskunde GD, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, Equine Internal Medicine, dES AVR, CS_Welfare & emerging diseases, Voortplanting paard, dES/dFAH FR, CS_Fertility |
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| Přispěvatelé: | Interne geneeskunde GD, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, Equine Internal Medicine, dES AVR, CS_Welfare & emerging diseases, Voortplanting paard, dES/dFAH FR, CS_Fertility |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Offspring
Veterinary medicine Glucose uptake Mare Biology Article Andrology Equine conceptus SF600-1100 Gene expression Conceptus Maternal nutrition Fetus General Veterinary urogenital system Insulin-like growth factor 2 receptor Embryo veterinary(all) Epigenetic reprogramming Nutrient transporters QL1-991 embryonic structures Animal Science and Zoology Genomic imprinting Zoology |
| Zdroj: | Animals, 11(9), 1. Multidisciplinary Digital Publishing Institute (MDPI) Animals : an Open Access Journal from MDPI Animals Volume 11 Issue 9 Animals, Vol 11, Iss 2504, p 2504 (2021) |
| ISSN: | 2076-2615 |
| Popis: | Simple Summary In pregnant individuals, maternal overnutrition is associated with disturbances in the expression of specific genes and nutrient transporters in the early embryo, which can affect both fetal and placental development and have lasting effects on the health of resulting offspring. To examine how maternal overfeeding affects the equine embryo, Shetland pony mares were fed either a high-energy (HE: 200% of net energy requirements) or maintenance (control) diet. Mares from both groups were inseminated, and day-seven embryos were recovered and transferred to recipients from the same or the alternate group. The expression of several genes, nutrient transporters and DNA methyltransferases (DNMTs; play an important role in regulating gene expression) were determined in extra-embryonic membranes after recovery on day 28 of gestation. The expression of nutrient transporters was also assessed in endometrium recovered from recipient mares immediately after embryo removal. In addition, glucose uptake by day-28 extra-embryonic membranes, and lipid droplet accumulation in day-seven embryos were assessed. Maternal overfeeding resulted in elevated expression of several genes, DNMTs and nutrient transporters following embryo transfer from an HE to a control mare. The expression of two amino acid transporters was also elevated in the endometrium after embryo transfer from HE to control. Maternal overfeeding did not affect lipid droplet accumulation in day-seven embryos, or glucose uptake by membranes of day-28 embryos. It remains to be seen whether the alterations in gene expression are maintained throughout gestation and into postnatal life. Abstract Maternal overfeeding is associated with disturbances in early embryonic epigenetic reprogramming, leading to altered expression of imprinted genes and nutrient transporters, which can affect both fetal and placental development and have lasting effects on the health of resulting offspring. To examine how maternal overfeeding affects the equine embryo, Shetland pony mares were fed either a high-energy (HE: 200% of net energy requirements) or maintenance (control) diet. Mares from both groups were inseminated, and day-seven embryos were recovered and transferred to recipients from the same or the alternate group. The expression of a panel of imprinted genes, glucose and amino acid transporters, and DNA methyltransferases (DNMTs) were determined in conceptus membranes after recovery on day 28 of gestation (late pre-implantation phase). The expression of nutrient transporters was also assessed in endometrium recovered from recipient mares immediately after conceptus removal. In addition, glucose uptake by day-28 extra-embryonic membranes, and lipid droplet accumulation in day-seven blastocysts were assessed. Maternal overfeeding resulted in elevated expression of imprinted genes (IGF2, IGF2R, H19, GRB10, PEG10 and SNRPN), DNMTs (DNMT1 and DNMT3B), glucose (SLC2A1), fructose (SLC2A5) and amino acid (SLC7A2) transporters following ET from an HE to a control mare. Expression of amino acid transporters (SLC1A5 and SLC7A1) was also elevated in the endometrium after ET from HE to control. Maternal overfeeding did not affect lipid droplet accumulation in blastocysts, or glucose uptake by day-28 membranes. It remains to be seen whether the alterations in gene expression are maintained throughout gestation and into postnatal life. |
| Databáze: | OpenAIRE |
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