Morphometric Analysis of Pulmonary Development in the Sheep Following Creation of Fetal Diaphragmatic Hernia
| Autor: | Jill Lipsett, S. C. Runciman, A. J. Martin, J. D. Kennedy, W. D. A. Ford, Roger W. Byard, Johanna C. Cool |
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| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Fetus business.industry Urology Congenital diaphragmatic hernia General Medicine medicine.disease Surgery Pathology and Forensic Medicine Pulmonary hypoplasia Morphometric analysis Parenchyma Pediatrics Perinatology and Child Health medicine Gestation Diaphragmatic hernia Lung volumes business |
| Zdroj: | Fetal and Pediatric Pathology. 17:789-807 |
| ISSN: | 1551-3815 |
| DOI: | 10.3109/15513819709168601 |
| Popis: | Congenital diaphragmatic hernia (CDH) in humans is relatively common and associated with high mortality attributed mainly to pulmonary hypoplasia. Previous animal models have induced CDH late in gestation, in contrast to the human situation, and only limited morphometric analyses have been reported. We undertook early surgical creation of CDH in fetal lambs, days 72–74 of gestation (n = 8), with unoperated lambs (n = 8) as controls. At 143 days (term = 145–149) a cesarean section was performed and the lungs were obtained, inflation fixed, divided into lobes, and processed for morphometry. In the CDH group the total lung volumes (51.3 mL compared to 223.8 mL) and gas exchange surface areas (5.85 m2 versus 26.43 m2) were less than one quarter of control values. Capillary loading was reduced from 0.3 mL/m2 in controls to 0.12 mL/m2 in CDH and parenchymal volume reduced from 77% in controls to 57% in CDH. Within parenchyma, gas exchange tissue volume was increased in CDH (66%) compared with controls (50%). CD... |
| Databáze: | OpenAIRE |
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