Immunogenicity of formaldehyde-inactivated enterotoxins A and C1 of Staphylococcus aureus
| Autor: | Joseph F. Metzger, Leonard Spero, John R. Warren, Virginia G. McGann |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Immunodiffusion
Staphylococcus Enterotoxin Biology medicine.disease_cause Microbiology Sepharose Enterotoxins Formaldehyde medicine Immunology and Allergy Animals Staphylococcus aureus delta toxin Antiserum Antigens Bacterial Toxin Immunogenicity Immune Sera Sodium Dodecyl Sulfate Hemagglutination Tests Hydrogen-Ion Concentration Chromatography Ion Exchange Macaca mulatta Infectious Diseases Sephadex Staphylococcus aureus Antibody Formation Chromatography Gel Electrophoresis Polyacrylamide Gel Rabbits |
| Zdroj: | The Journal of infectious diseases. 131(5) |
| ISSN: | 0022-1899 |
| Popis: | Quantitative precipitation of antisera specific for native enterotoxin revealed that 70% and 60%, respectively, of the antigenic determinants of enterotoxins A and C1 of Staphylococcus aureus were inactivated by formaldehyde at pH 5.0 or 7.5 contained large polymers (excluded by Sepharose 2B) and induced strong humoral immune responses in rhesus monkeys. Enterotoxin A inactivated at pH 5.0 or 7.5 was composed mostly of small polymers (excluded by Sephadex G-100 but included by Sepharose 2B); it provoked a poor immune response in monkeys (about equivalent to the response obtained with weakly reactive toxin inactivated at alkaline pH). It was concluded that potent enterotoxoids were formed by extensive cross-linking of enterotoxin C1 into large polymers in acidic or neutral formaldehyde solution. |
| Databáze: | OpenAIRE |
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