Production of antikeratin autoantibodies by hybrid spleen cells of naive mice
| Autor: | Masahiro Takigawa, Mizuho Yamada, K Sugaya, Keiji Iwatsuki, Shunsuke Imaizumi, Hideo Hashizume |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
T-Lymphocytes
Blotting Western Immunoblotting Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Spleen Dermatology Hybrid Cells Biology Mice Western blot Antigen Keratin medicine Animals Humans Autoantibodies chemistry.chemical_classification Mice Inbred BALB C integumentary system medicine.diagnostic_test Autoantibody Molecular biology medicine.anatomical_structure Immunization chemistry biology.protein Keratins Immunohistochemistry Epidermis Antibody |
| Zdroj: | British Journal of Dermatology. 123:735-744 |
| ISSN: | 1365-2133 0007-0963 |
| Popis: | The mechanism of the occurrence of natural antikeratin antibodies in human sera was studied using hybrid spleen cells obtained from experimentally naive or from immunized mice. Antikeratin antibodies were detected by enzyme-linked immunosorbent assay (ELISA) in 5.9-9.5% of the culture supernatants of fused spleen cells taken from naive mice. When mice were immunized with keratins, the number of supernatants containing antikeratin antibodies was increased to eight out of 51 (15.7%). When immunized with non-keratin materials such as activated human T cells, adult T-cell leukaemia cell lysates, and human T-cell lymphotropic virus type-I (HTLV-I), 16.7-20.8% of the supernatants were found to contain antikeratin antibodies by ELISA. The antikeratin antibodies in the supernatants showed cytoplasmic staining of keratinocytes in human as well as mouse skin by indirect immunofluorescence. The antibodies reacted with extracted human epidermal keratins by dot-blot and Western blot analysis. Most antikeratin antibodies in the supernatants did not show cross-reactivity with exogenous antigens used for immunization and vimentin-type intermediate-sized filaments. These findings demonstrate that B cells producing antikeratin antibodies are common in naive mice, and produce various types of antikeratin antibodies following specific activation with epidermal keratins and non-specific immunological stimuli. |
| Databáze: | OpenAIRE |
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