Expression of the human cytomegalovirus genome in mouse cells and in human-mouse heterokaryons
| Autor: | Eva Gonczol, G. Váczi, Istvan Boldogh, L. Gärtner |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Human cytomegalovirus
Cytoplasm Cytomegalovirus Hybrid Cells Biology Virus Replication Genome Virus Cell Line Cell Fusion Mice Antigen Idoxuridine Virology medicine Animals Humans Fibroblast Antigens Viral Cell Nucleus Heterokaryon fungi General Medicine medicine.disease Embryonic stem cell Parainfluenza Virus 1 Human medicine.anatomical_structure Genes |
| Zdroj: | Archives of Virology. 53:101-108 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01314851 |
| Popis: | Mouse cells with an established human cytomegalovirus (HCMV) infection were fused with susceptible human embryonic fibroblast cells. CMV-specific early antigens could be demonstrated in the cytoplasm and cell-membrane of the heterokaryons. Treatment with 5-iodo-2'-deoxyuridine (IUdR) of the heterokaryons or of the latently infected mouse cells, prior to their fusion with human cells, could induce the appearance of immunoflourescent elements, characterised as late antigens, and of infectious virus. Our data show that the mouse cells, in the latent stage of infection, contain the whole virus genome and that the replication of the virus is controlled by a genetic mechanism of the host cells both in virus-harbouring mouse cells and in heterokaryons. |
| Databáze: | OpenAIRE |
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