Description of inflammation and cytokine profile at the inoculation site and in heart tissue of mice re-infected with Trypanosoma cruzi vector derived-metacyclic trypomastigotes
| Autor: | R. Alejandre-Aguilar, Victor M. Monteón, A. Aranda-Fraustro, R. Lopez, L. Gómez-García |
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| Rok vydání: | 2005 |
| Předmět: |
Chagas disease
medicine.medical_treatment Apoptosis Dermatitis Inflammation Biology Parasitemia Mice In Situ Nick-End Labeling medicine Animals Chagas Disease Vector (molecular biology) Trypanosoma cruzi Lymph node Skin Mice Inbred BALB C Inoculation Myocardium biology.organism_classification medicine.disease Infectious Diseases Cytokine medicine.anatomical_structure Immunology Cytokines Female Animal Science and Zoology Parasitology Lymph Nodes Lymph medicine.symptom |
| Zdroj: | Parasitology. 130:511-522 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182004006833 |
| Popis: | We studied the role of Trypanosoma cruzi reinfection in regard to inflammatory and cytokine response at the inoculation site, lymph node and heart. We reinfected Balb/c mice intradermically into the hind foot-pad with natural infective metacyclic trypomastigotes. They were followed from 24 h to 30 days after the last reinfection. At the inoculation site 24 h after the last re-infection, the infiltrating inflammatory cells increased dramatically with respect to baseline inflammation, reaching maximum infiltrates for the third day. In contrast, parasite DNA was undetectable 24 h after inoculation, despite poor cytokine induction, only IFN-gamma, IL-12 and TGF-beta were noticeable on days 7 and 15, whereas in the lymph nodes draining the inoculation site positive expression of IL-2, IL-4, IL-12 and TGF-beta were found to be induced as soon as 24 h after re-entry of parasite. In the heart, the inflammatory response increased immediately 24 h after re-entry of parasites, reaching its maximum on the 7th day and returning to baseline on day 30. In conclusion, although the inflammatory response is triggered in both compartments by re-entry of parasites, the inflammatory process returns almost to baseline after 30 days, leaving a persistent low-grade inflammation. |
| Databáze: | OpenAIRE |
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