Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis
| Autor: | Marie Josée Rocheleau, Norman J. Dovichi, Da Yong Chen, Ronda. Grey, Heather R. Harke |
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| Rok vydání: | 1992 |
| Předmět: |
Formamide
Gel electrophoresis Chromatography Gel electrophoresis of nucleic acids Base Sequence Formamides Clinical Biochemistry Polyacrylamide Temperature DNA Nucleic Acid Denaturation Biochemistry Analytical Chemistry Electrophoresis chemistry.chemical_compound Capillary electrophoresis chemistry Molecular-weight size marker Pulsed-field gel electrophoresis Electrophoresis Polyacrylamide Gel Gels |
| Zdroj: | Electrophoresis. 13(8) |
| ISSN: | 0173-0835 |
| Popis: | Compressions are occasionally found during the separation of DNA sequencing fragments, particularly in G/C-rich regions and in gels operated at room temperature. Addition of at least 10% formamide to urea/polyacrylamide sequencing gels improves the denaturing capacity of the gel, minimizing compressions. Addition of 20% or more formamide decreases the separation rate, theoretical plate count, and resolution for normally migrating fragments. An optimum concentration of 10% formamide improves resolution of compressed regions without degrading the other characteristics of the gel. Operation of gels at room temperature simplifies the engineering associated with automated sequencers based on capillary gel electrophoresis. |
| Databáze: | OpenAIRE |
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