Circulating TFH cells is correlated with disease activity in anti-MDA5 antibody positive idiopathic inflammatory myopathies

Autor: Mianjing, Zhou, Mengyuan, Li, Chaohuan, Guo, Shuang, Wang, Xue, Li, Siyuan, Zhao, Hui, Zhang, Niansheng, Yang
Rok vydání: 2021
Předmět:
Zdroj: Clinical and Experimental Rheumatology. 39:804-810
ISSN: 1593-098X
0392-856X
DOI: 10.55563/clinexprheumatol/gfqlcz
Popis: Idiopathic inflammatory myopathies (IIM) are a group of disorders characterised by the production of autoantibodies and inflammatory infiltrates in the skeletal muscles. Follicular T helper (TFH) cells are known to be crucial for B cell differentiation and autoantibody production in autoimmune diseases. The aim of this study was to investigate the involvement of TFH cells in IIM.Circulating TFH cells in 44 IIM patients or 11 age- and gender-matched healthy controls (HCs) were measured by flow cytometry. ICOS, PD-1, active caspase-1 and Ki-67 expression in TFH cells was examined. The correlations between the frequency of TFH cells and clinical disease activities were also analysed.The frequency of TFH cells was 16.6% in IIM patients with anti-melanoma differentiation-associated gene (MDA5) antibody compared to 10.6% and 12.9% in anti-MDA5 negative patients or HCs, respectively (both p0.05). The frequency of TFH cells was positively correlated with clinical disease activities: patient/parent's assessment VAS (r=0.51, p0.05), physician's assessment VAS (r=0.59, p0.05) and MYOACT scores (total systems: r=0.62, p0.05; extramuscular system: r=0.56, p0.05; pulmonary system, r=0.55, p0.05). The percentage of PD-1highICOShigh TFH cells was 3.68% in anti-MDA5 positive patients compared to 2.70% and 1.96% in anti-MDA5 negative patients or HCs, respectively (both p0.05). The percentage of Ki-67 positive TFH cells was 3.50% in anti-MDA5 positive patients compared to 2.36% and 1.76% in anti-MDA5 negative patients or HCs, respectively (p0.05). Interestingly, active caspase-1 was significantly increased in TFH cells in anti-MDA5 positive patients compared to the patients without anti-MDA5 or HCs (3.30% vs. 1.67% and 3.30% vs. 1.02%, both p0.001).These data suggest a role for TFH cells in the pathogenesis of anti-MDA5 positive IIM and TFH cells might serve as a disease biomarker for this subset of patients.
Databáze: OpenAIRE
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