Identification of molecular compartments and genetic circuitry in the developing mammalian kidney
| Autor: | Joe E. Vaughan, David H. Rowitch, Karl Staser, Rathi D Thiagarajan, Alexander van Oudenaarden, Jing Yu, Qun Ren, Melissa H. Little, Charles D. Stiles, Philip Machanick, Qiufu Ma, A. Michaela Krautzberger, Kylie Georgas, Mary Duah, Bree Rumballe, Andrew P. McMahon, Jinjin Guo, Jan Philipp Junker, Jennifer K. Hansard, M. Todd Valerius, Jill A. McMahon, Paul A. Gray, Timothy L. Bailey, Sean M. Grimmond, Diane Faria |
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| Rok vydání: | 2012 |
| Předmět: |
Cell type
Cellular differentiation Cell Urogenital System Computational biology Biology Kidney 03 medical and health sciences Mice 0302 clinical medicine medicine Transcriptional regulation Animals Molecular Biology In Situ Hybridization Research Articles 030304 developmental biology Regulation of gene expression Genetics 0303 health sciences Gene Expression Profiling Kidney metabolism Gene Expression Regulation Developmental 3. Good health Gene expression profiling medicine.anatomical_structure Developmental biology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England). 139(10) |
| ISSN: | 1477-9129 |
| Popis: | Lengthy developmental programs generate cell diversity within an organotypic framework, enabling the later physiological actions of each organ system. Cell identity, cell diversity and cell function are determined by cell type-specific transcriptional programs; consequently, transcriptional regulatory factors are useful markers of emerging cellular complexity, and their expression patterns provide insights into the regulatory mechanisms at play. We performed a comprehensive genome-scale in situ expression screen of 921 transcriptional regulators in the developing mammalian urogenital system. Focusing on the kidney, analysis of regional-specific expression patterns identified novel markers and cell types associated with development and patterning of the urinary system. Furthermore, promoter analysis of synexpressed genes predicts transcriptional control mechanisms that regulate cell differentiation. The annotated informational resource (www.gudmap.org) will facilitate functional analysis of the mammalian kidney and provides useful information for the generation of novel genetic tools to manipulate emerging cell populations. |
| Databáze: | OpenAIRE |
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