Functional role of glycosphingolipids in contact inhibition of growth in a human mammary epithelial cell line
| Autor: | Kazuko Handa, Sen-itiroh Hakomori, Nathan Schurman, Xiaohua Huang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biophysics Cell Count Biology Biochemistry Glycosphingolipids law.invention 03 medical and health sciences Structural Biology law Cell Line Tumor Genetics medicine Ganglioside GD3 Humans RNA Messenger RNA Small Interfering Mammary Glands Human Molecular Biology Cell Proliferation Galactosyltransferase Cell growth Contact Inhibition Contact inhibition Cell Biology Galactosyltransferases Epithelium Sialyltransferases Cell biology Merlin (protein) 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Gene Knockdown Techniques Suppressor lipids (amino acids peptides and proteins) Function (biology) |
| Zdroj: | FEBS letters. 591(13) |
| ISSN: | 1873-3468 |
| Popis: | We have demonstrated previously the involvement of certain glycosphingolipids (GSLs) in 'contact inhibition' (dependent on cell-to-cell contact) of cell growth. Here, we examined the roles of specific GSLs in contact inhibition of the human epithelial cell line MCF10A. Contact-inhibited cells show increased expression of the ganglioside GD3 and the globo-series GSL Gb3, and of the mRNAs for the corresponding sialyltransferases ST8SIA1 (GD3 synthase) and galactosyltransferase A4GALT (Gb3 synthase). siRNA knockdown (KD) of ST8SIA1 and/or A4GALT significantly suppresses contact inhibition. Exogenous addition of GD3 or Gb3 inhibits proliferation of low-density cells. Our findings suggest that GSLs play functional roles in contact inhibition of these cells and that Merlin/NF2, a tumor suppressor protein, is involved in the GSL function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text