Increased urothelial paracellular transport promotes cystitis
| Autor: | Nicolas Montalbetti, Amity F. Eaton, H. Sandeep Prakasam, Sheldon I. Bastacky, Marcelo D. Carattino, Anna C. Rued, Gerard Apodaca, Wily G. Ruiz, F. Aura Kullmann, Dennis R. Clayton |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Membrane Permeability Physiology Inflammation Biology Tight Junctions Green fluorescent protein Rats Sprague-Dawley Lymphocytic Infiltrate In vivo Edema Cystitis medicine Animals Urinary bladder Tight junction Epithelial Cells Muscle Smooth Articles medicine.anatomical_structure Paracellular transport Claudins Female Urothelium medicine.symptom |
| Zdroj: | American Journal of Physiology-Renal Physiology. 309:F1070-F1081 |
| ISSN: | 1522-1466 1931-857X |
| Popis: | Changes in the urothelial barrier are observed in patients with cystitis, but whether this leads to inflammation or occurs in response to it is currently unknown. To determine whether urothelial barrier dysfunction is sufficient to promote cystitis, we employed in situ adenoviral transduction to selectively overexpress the pore-forming tight junction-associated protein claudin-2 (CLDN-2). As expected, the expression of CLDN-2 in the umbrella cells increased the permeability of the paracellular route toward ions, but not to large organic molecules. In vivo studies of bladder function revealed higher intravesical basal pressures, reduced compliance, and increased voiding frequency in rats transduced with CLDN-2 vs. controls transduced with green fluorescent protein. While the integrity of the urothelial barrier was preserved in the rats transduced with CLDN-2, we found that the expression of this protein in the umbrella cells initiated an inflammatory process in the urinary bladder characterized by edema and the presence of a lymphocytic infiltrate. Taken together, these results are consistent with the notion that urothelial barrier dysfunction may be sufficient to trigger bladder inflammation and to alter bladder function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|