Platelet-activating factor (PAF-acether) induced leukotriene C4 formation and luminol dependent chemiluminescence by human eosinophils
| Autor: | Leo Koenderman, Jan Verhagen, Pieter L.B. Bruynzeel, P.T.M. Kok, M.L. Hameling |
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| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_element
In Vitro Techniques Calcium law.invention Luminol chemistry.chemical_compound law Humans Platelet Activating Factor Mode of action Chemiluminescence Pharmacology Platelet-activating factor Leukotriene C4 Chemistry Zymosan Antagonist respiratory system Eosinophils N-Formylmethionine Leucyl-Phenylalanine Pyridazines Biochemistry Luminescent Measurements Biophysics SRS-A lipids (amino acids peptides and proteins) |
| Zdroj: | Pharmacological Research Communications. 18:61-69 |
| ISSN: | 0031-6989 |
| DOI: | 10.1016/0031-6989(86)90039-1 |
| Popis: | Human eosinophils are capable of synthesizing almost exclusively the strongly spasmogenic compound LTC4 when stimulated with either the calcium ionophore A 23187 or opsonized zymosan (OZ). Although PAF-acether in concentrations ranging from 10 nM to 1 microM is hardly capable of inducing significant LTC4 synthesis itself, it significantly enhances the OZ-induced LTC4 formation at a concentration of 1 microM. However, at a concentration of 10 microM, PAF-acether itself is capable of inducing LTC4 formation comparable with that induced by OZ. PAF-acether, at a concentration of 10 microM (and not at a concentration of 1 microM) is also capable of inducing a luminol dependent chemiluminescent response by eosinophils. The PAF-acether antagonist BN 52021 at a concentration of 0.1 mM not only partially inhibited the PAF-acether induced LTC4 formation but also the OZ induced LTC4 formation. Since an equal inhibition is found the inhibitory mode of action of BN 52021 is most likely directed towards a common pathway. Taken together, these results suggest that eosinophils may be triggered by high locally reached concentrations of PAF-acether to release inflammatory and bronchoconstrictive mediators. This may be of importance for the pathogenesis of the allergen induced late phase asthmatic reaction. |
| Databáze: | OpenAIRE |
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