Polarized distribution of renal natriuretic peptide receptors in normal physiology and ischemia
| Autor: | Alan D. Dean, D. Ritter, James E. Greenwald, Zhonghong Guan |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Physiology medicine.drug_class Urinary system Renal cortex Ischemia Receptors Cell Surface Biology Kidney Cell Line Renal Circulation Mice Atrial natriuretic peptide Reference Values Internal medicine medicine Natriuretic peptide Animals Tissue Distribution Receptor Cell Polarity Brain natriuretic peptide medicine.disease Rats medicine.anatomical_structure Endocrinology Guanylate Cyclase Natriuretic Agents |
| Zdroj: | The American journal of physiology. 269(6 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | The polarized expression of guanylyl cyclase-coupled natriuretic peptide receptors, types A (GC-A) and B (GC-B), was measured in inner medullary collecting ducts (IMCD) of normal and ischemic rat kidneys, as well as in IMCD cells. Exposure of normal rat kidney medulla to an anti-GC-A antibody demonstrated a propensity of receptor staining on the cellular basal membrane. The polarization of GC-A receptors was lost in the ischemic kidney. The maximal binding capacity of 125I-atrial natriuretic factor (ANF) to the basal membrane of the inner medullary cell line mIMCD-K2 was five times greater than that to the apical membrane. ANF or C-type natriuretic peptide (CNP) added to the basal side of cultured cells resulted in guanosine 3',5'-cyclic monophosphate formation that was greater than when applied to the apical side. Depletion of ATP stores in cultured cells was followed by an increase of 125I-ANF binding to apical cellular membranes. Similar results were obtained when receptor guanylyl cyclase activity was assayed. In conclusion, these results suggest that functional GC-A and GC-B receptors are present predominantly on the basal membrane of IMCD. However, depletion of cellular ATP stores such as in ischemia is followed by a partial loss of polarization. |
| Databáze: | OpenAIRE |
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