| Popis: |
Multidrug-resistant organisms have increased the prevalence of infectious diseases and have become the leading source of death globally. The adverse effects associated with conventional antibiotics cannot be underestimated, and as a result, the quest for antibacterial agents has received great attention over the years. Therefore, the current research was designed to synthesize and examine the antibacterial properties of two metallic nanoparticles, silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs), as well as their antibiotic combination therapy against multidrug-resistant bacteria. AgNPs and ZnONPs were synthesized by the coprecipitation method and characterized. Thereafter, their antibacterial activity against multidrug-resistant bacteria was investigated using the microdilution technique. Subsequently, the interactions between the synthesized nanoparticles and antibiotics were evaluated by checkerboard assay. Time-kill assays were carried out to assess bacteriostatic or bactericidal effects, and the cytotoxicity study was carried out by MTT assay. The SEM analysis of AgNPs and ZnONPs were spherical with an average size of 21.03 and 43.37 nm, respectively. FTIR analysis showed the characteristics of the metal-oxygen vibrational band for both materials around 450 cm |
