| Popis: |
The nanoparticles have been recognized to play vital coating roles in electronics, energy contact actions, biology, and medicine due to their unique properties for two decades. In the present study, silver nanoparticles were synthesized using an endophytic fungus, Aspergillus aureoles, and subjected to antimicrobial activity. The treatment of an aqueous solution of silver nitrate (3 mM) with cell-free filtrate of Aspergillus aureoles resulted in the production of stable and crystalline silver nanoparticles. Silver nanoparticles were characterized using different studies, such as Ultraviolet-Visible Spectroscopy, High-Resolution Transmission Electron Microscopy, X-ray Diffraction Spectroscopy, Fourier Transform Infrared Spectroscopy, and Dynamic Light Scattering. The antimicrobial activity was carried out by the well diffusion method. The nanoparticles were in a spherical shape with an average particle size of 12-15 nm as observed in a High-Resolution Transmission Electron Microscope. The crystalline nature and size of nanoparticles were confirmed by the X-ray Diffraction Spectroscopy and Selected Area Electron Diffraction, respectively. The Fourier Transform Infrared Spectroscopic study revealed the involvement of various functional groups of Aspergillus aureoles extract in stabilizing the nanoparticles. These nanoparticles exhibited potent antimicrobial activity against both gram-positive and negative bacteria, which was comparable to the standard antibiotic. Thus, the study established the possible use of green silver nanoparticles in controlling microbial infections and developing a novel broad-spectrum antimicrobial agent/s. |
