Effects of tumour necrosis factor-alpha and interleukin-1 beta on in vitro development of bovine secondary follicles
| Autor: | Bruno G Matos-Brito, E.V. Cunha, M.A.M. Donato, Glaucinete B Souza, A. W. B. Silva, Robert van den Hurk, Cristina A Peixoto, Lais R F M Paulino, José Vieira Silva, Ewerton P F Lopes |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ovarian Cortex medicine.medical_treatment Interleukin-1beta Growth Differentiation Factor 9 Histones Tissue Culture Techniques Andrology 03 medical and health sciences Endocrinology Ovarian Follicle Follicular phase medicine Animals Cyclin B1 Antrum Tumor Necrosis Factor-alpha Chemistry In vitro 030104 developmental biology Cytokine Gene Expression Regulation Proto-Oncogene Proteins c-mos Ultrastructure Cattle Female Animal Science and Zoology Tumor necrosis factor alpha Folliculogenesis Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 53:997-1005 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.13199 |
| Popis: | The objective of this study was to determine the effects of TNF-α and IL-1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO2 in air, for 18 days, in TCM-199+ alone (cultured control) or supplemented with 10 ng/ml IL-1β, 10 ng/ml TNF-α or both TNF-α and IL-1β. The effects of these treatments on growth, follicular survival, antrum formation, viability, ultrastructure and mRNA levels for GDF-9, c-MOS, H1foo and Cyclin B1 were evaluated. The results showed that addition of TNF-α to culture medium increased follicular diameter and rate of antrum formation, whereas that of IL-1β and a mixture of IL-1β and TNF-α did not do so. Ultrastructural analysis showed that, among the tested cytokine treatments, follicles cultured in the presence of TNF-α had the best-preserved oocytes and granulosa cells. The presence of TNF-α, IL-1β or both did not influence the expression of mRNAs analysed. In conclusion, in contrast to IL-1β, TNF-α promotes growth of and antrum formation in in vitro cultured bovine secondary follicles, while their ultrastructure and viability were maintained. |
| Databáze: | OpenAIRE |
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