Potentiation of synaptic transmission in Rat anterior cingulate cortex by chronic itch

Autor: Ting-Ting Zhang, Bin Wang, Zhi-Ru Wang, Wen Wen, Xuan Zhao, Li-qing Ma, Feng-Yan Shen, Yuan-Zhi Peng
Rok vydání: 2016
Předmět:
Cyclopropanes
0301 basic medicine
N-Methylaspartate
AMPA receptor
Neurotransmission
Gyrus Cinguli
Synaptic Transmission
behavioral disciplines and activities
Anterior cingulate cortex
Rats
Sprague-Dawley
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Postsynaptic potential
medicine
Animals
Silent synapse
Receptors
AMPA
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Molecular Biology
Chronic itch
Neurons
Behavior
Animal
Chemistry
Research
Pruritus
Excitatory Postsynaptic Potentials
Long-term potentiation
Up-Regulation
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
nervous system
Chronic Disease
Excitatory postsynaptic potential
NMDA receptor
Neuroscience
psychological phenomena and processes
030217 neurology & neurosurgery
Zdroj: Molecular Brain
ISSN: 1756-6606
DOI: 10.1186/s13041-016-0251-1
Popis: Itch and pain share similar mechanisms. It has been well documented that the anterior cingulate cortex (ACC) is important for pain-related perception. ACC has also been approved to be a potential pruritus-associated brain region. However, the mechanism of sensitization in pruriceptive neurons in the ACC is not clear. In current study, a chronic itch model was established by diphenylcyclopropenone (DCP) application. We found that both the frequency and amplitude of miniature excitatory postsynaptic currents in the ACC were enhanced after the formation of chronic itch. The paired-pulse ratio in ACC neurons recorded from the DCP group were smaller than those recorded in control group at the 50-ms interval. We also observe a significant increase in the AMPA/NMDA ratio in the DCP group. Moreover, an increased inward rectification of AMPARs in ACC pyramidal neurons was observed in the DCP group. Interestingly, the calculated ratio of silent synapses was significantly reduced in the DCP group compared with controls. Taken together, we conclude that a potentiation of synaptic transmission in the ACC can be induced by chronic itch, and unsilencing silent synapses, which probably involved recruitment of AMPARS, contributed to the potentiation of postsynaptic transmission.
Databáze: OpenAIRE
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