The protective effects of rapamycin on cell autophagy in the renal tissues of rats with diabetic nephropathy via mTOR-S6K1-LC3II signaling pathway
| Autor: | Lijuan Yang, Jiqiang Zhang, Xiangdong Yang, Yaling Guo, Baochao Chang, Lei Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male autophagy podocyte P70-S6 Kinase 1 Diabetic nephropathy Critical Care and Intensive Care Medicine lcsh:RC870-923 Streptozocin Podocyte Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences Laboratory Study Medicine Animals Diabetic Nephropathies PI3K/AKT/mTOR pathway Sirolimus business.industry Podocytes rapamycin Ribosomal Protein S6 Kinases TOR Serine-Threonine Kinases Autophagy Intracellular Signaling Peptides and Proteins Glomerulosclerosis Membrane Proteins General Medicine medicine.disease lcsh:Diseases of the genitourinary system. Urology Rats Proteinuria 030104 developmental biology medicine.anatomical_structure Nephrology Creatinine Cancer research mTOR Signal transduction business Signal Transduction |
| Zdroj: | Renal Failure, Vol 40, Iss 1, Pp 492-497 (2018) Renal Failure |
| ISSN: | 1525-6049 |
| Popis: | Background: Previous studies have shown that podocyte autophagy is an important trigger for proteinuria and glomerulosclerosis. The mammalian rapamycin target protein (mTOR) occupies a pivotal position in the autophagy pathway. In this study, we planned to clarify the mechanism of mTOR regulation of podocyte autophagy and the effect of rapamycin (RAPA). Methods: All rats were randomly divided into normal control group (n = 8), DN group (n = 8), and RAPA group (n = 8). Blood and urine samples were collected at the 4th, 8th, and 12th weeks of the experiment. The serum creatinine (Scr), urine volume levels, and the 24 h urine protein (UP) levels were examined. The nephrin, podocin, mTOR, ribosomal S6 kinase 1 (S6K1), and autophagy marker light chain 3 (LC3II) expression levels were evaluated by immunohistochemistry, quantitative PCR, and immunoblotting. Results: The urine volume, 24 h UP, and Scr of the DN and RAPA groups increased significantly compared with the NC group (p |
| Databáze: | OpenAIRE |
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