Simultaneous determination of cysteine sulfinic acid and hypotaurine in rat tissues by column-switching high-performance liquid chromatography with electrochemical detection
| Autor: | Koji Kawanabe, Atsushi Ohsawa, Tadayasu Togawa, Shinzo Tanabe |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Taurine Hypotaurine Electrochemical detection Sulfinic acid Kidney High-performance liquid chromatography chemistry.chemical_compound Electrochemistry Animals Column switching Cysteine Rats Wistar Electrodes Chromatography High Pressure Liquid Brain Chemistry chemistry.chemical_classification Neurotransmitter Agents Chromatography General Chemistry Carbon Rats Liver chemistry Cysteine sulfinic acid Cyclic voltammetry Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 704:83-88 |
| ISSN: | 0378-4347 |
| Popis: | Cysteine sulfinic acid (CSA) and hypotaurine (HT) were determined by electrochemical detection with a glassy carbon electrode at 0.95 V vs. Ag/AgCl. The separation of CSA and HT was accomplished by coupled-column liquid chromatography, consisting of an anion-exchange column and a cation-exchange column. For the determination of CSA and HT in rat tissues, a column-switching system was introduced to remove interferences from late-eluting endogenous substances. The limits of determination were 0.05 microM for both sulfinic acids. The average precisions (C.V.) over the concentration range of 0.05 to 5 microM were 4.3% for CSA and 4.2% for HT. |
| Databáze: | OpenAIRE |
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