Fluorescent europium-modified polymer nanoparticles for rapid and sensitive anthrax sensors
| Autor: | Jooyoung Song, Jyongsik Jang, Wan-Kyu Oh, Yoon Seon Jeong |
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| Rok vydání: | 2011 |
| Předmět: |
Acrylic Resins
Biomedical Engineering Biophysics Metal Nanoparticles chemistry.chemical_element Nanoparticle Nanotechnology Biosensing Techniques chemistry.chemical_compound Europium Limit of Detection Spectroscopy Fourier Transform Infrared Electrochemistry Particle Size Picolinic Acids Fluorescent Dyes Spores Bacterial Aqueous solution biology Polyacrylonitrile General Medicine biology.organism_classification Dipicolinic acid Fluorescence Bacillus anthracis Polymerization chemistry Microscopy Electron Scanning Biotechnology Nuclear chemistry |
| Zdroj: | Biosensors and Bioelectronics. 29:172-177 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2011.08.013 |
| Popis: | Novel fluorescent polyacrylonitrile nanoparticles were synthesized by microemulsion polymerization and Schiff base modification. By further modification with europium, the polyacrylonitrile nanoparticles could be used as a highly sensitive and rapid sensor for Bacillus anthracis spore detection in aqueous solution. The europium-modified polyacrylonitrile nanoparticles were readily combined with dipicolinic acid as a unique biomarker of B. anthracis , leading to high fluorescence emission. These nanoparticles enabled ratiometric detection without instrument-specific calibration due to the internal fluorescence reference. Additionally, the europium-modified polyacrylonitrile nanoparticle sensors exhibited a remarkable limit of detection (10 pM) for dipicolinic acid and outstanding selectivity (160×) over aromatic ligands in aqueous solution. The ultrafine nanoparticle sensor showed a high capability for detecting anthrax due to the increased surface area-to-volume ratio and enhanced dispersibility. |
| Databáze: | OpenAIRE |
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