Assessing Genotype-By-Environment Interactions in Aspergillus Ear Rot and Pre-Harvest Aflatoxin Accumulation in Maize Inbred Lines
Autor: | Abigael Ouko, Henry Sila, Sheila Okoth, Altus Viljoen, Lindy J. Rose, Nakisani E. I. Netshifhefhe |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
Aflatoxin Veterinary medicine aflatoxins Aspergillus ear rot Aspergillus flavus colonization GGE biplot Aspergillus flavus 01 natural sciences AMMI stability index lcsh:Agriculture Inbred strain Genotype Colonization Gene–environment interaction Aspergillus environment GGI biology lcsh:S food and beverages Ammi 04 agricultural and veterinary sciences biology.organism_classification 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Agronomy, Vol 7, Iss 4, p 86 (2017) Agronomy, 7(4):86 Agronomy; Volume 7; Issue 4; Pages: 86 |
ISSN: | 2073-4395 |
Popis: | Aspergillus flavus, causal agent of the Aspergillus ear rot (AER) of maize, also produces aflatoxins that cause aflatoxicosis in humans and livestock. Ten maize inbred lines were evaluated in replicated trials in two aflatoxicosis outbreak hot spots in Kenya and in three maize-growing areas in South Africa for resistance to AER, A. flavus colonization, and pre-harvest aflatoxin accumulation during the 2012/13 growing season. AER severity was measured by visual assessment, while A. flavus colonization and aflatoxin content were quantified by real-time polymerase chain reaction (PCR) and liquid chromatography tandem mass spectrometry, respectively. Genotype by environment interaction (GEI) was determined using analysis of variance (ANOVA), additive main effects and multiplicative models (AMMI), and genotype plus by environment (GGE) biplot analyses. Stability of genotypes was evaluated using AMMI analysis. AER severity and fungal colonization significantly (p < 0.001) varied between genotypes. GEI influenced the severity of AER symptoms and aflatoxin accumulation significantly (p < 0.001), while fungal colonization was not affected. The inbred lines response was consistent for this trait in the test environments and was thus considered a desirable measure to indicate maize lines with a high risk of aflatoxin accumulation. CML495, CKL05019, LaPosta, and MIRTC5 were the least diseased lines, with the lowest aflatoxin contamination and a stable phenotypic response across the environments. Kiboko was determined as the ideal representative test environment, with discriminative ability of the genotypes for selection of the desired stable responses of the three traits. |
Databáze: | OpenAIRE |
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