Babesia canis spp. in dogs in Baghdad Province, Iraq: First molecular identification and clinical and epidemiological study
| Autor: | Afaf Abdulrahman Yousif, Naseir Mohammed Badawi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Tick infestation
Veterinary medicine medicine.medical_specialty 040301 veterinary sciences Prevalence SF1-1100 18S ribosomal RNA 030308 mycology & parasitology law.invention 0403 veterinary science 03 medical and health sciences law Epidemiology SF600-1100 medicine Polymerase chain reaction 0303 health sciences iraq General Veterinary biology Babesiosis baghdad 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Animal culture Canis babesia canis dog Babesia canis 18s rrna gene Research Article |
| Zdroj: | Veterinary World, Vol 13, Iss 3, Pp 579-585 (2020) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Aim: The aim of this study was to investigate babesiosis in dogs of different breeds and ages and of both sexes in Baghdad Province by molecular detection of Babesia canis using conventional polymerase chain reaction (PCR) and sequencing followed by phylogenetic analyses. Materials and Methods: Blood samples were collected from 310 dogs of different ages and breeds, and of both sexes in different areas of Baghdad Province from December 2018 to September 2019; during clinical examinations, body temperature, pulse, respiratory rate, and signs of diseases were recorded. PCR was used to amplify a specific 450-bp fragment of the 18S rRNA gene of B. canis. PCR products were sequenced, and MEGA 6.0 software was used for analysis. Chi-square and odds ratio tests were used to investigate the prevalence and risk factors of babesiosis. Results: Clinical signs of babesiosis included paleness or icterus of the mucus membranes, tick infestation, and febrile illness during the acute and subacute phase. The prevalence of infection with B. canis was 5.1%, with the higher prevalence in male dogs and in dogs |
| Databáze: | OpenAIRE |
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