Conformational Nanobodies Reveal Tethered Epidermal Growth Factor Receptor Involved in EGFR/ErbB2 Predimers
| Autor: | Daniel Baty, Benjamin Lombard, Damien Nevoltris, Elodie Dupuis, Patrick Chames, Gérard Mathis |
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| Přispěvatelé: | Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
MESH: Protein Structure Quaternary Receptor ErbB-2 General Physics and Astronomy Biosensing Techniques Epitopes Mice Antibody Specificity MESH: Animals General Materials Science ERBB3 Epidermal growth factor receptor conformational changes ERBB4 biology MESH: Protein Multimerization General Engineering Ligand (biochemistry) epidermal growth factor receptor (EGFR) nanobodies ErbB Receptors MESH: Receptor ErbB-2 Transmembrane domain Biochemistry MESH: Camelids New World phage display Camelids New World Tyrosine kinase MESH: Biosensing Techniques Binding domain MESH: Epitopes homogenous time-resolved fluorescence MESH: Receptor Epidermal Growth Factor MESH: Single-Domain Antibodies Cell Line single domain antibodies Animals Humans MESH: Antibody Specificity Protein Structure Quaternary MESH: Mice Binding Sites MESH: Humans Single-Domain Antibodies biosensors MESH: Cell Line MESH: Binding Sites Protein kinase domain biology.protein Biophysics Protein Multimerization |
| Zdroj: | ACS Nano ACS Nano, American Chemical Society, 2015, 9 (2), pp.1388-99. ⟨10.1021/nn505752u⟩ ACS Nano, 2015, 9 (2), pp.1388-99. ⟨10.1021/nn505752u⟩ |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | International audience; The epidermal growth factor receptor (EGFR) is a cell-surface receptor with a single transmembrane domain and tyrosine kinase activity carried by the intracellular domain. This receptor is one of the four members of the ErbB family including ErbB2, ErbB3, and ErbB4. Ligand binding, like EGF binding, induces a conformational rearrangement of the receptor and induces a homo/hetero dimerization essentially with ErbB family receptors that leads to the phosphorylation of the kinase domain, triggering a signaling cascade. EGFR can also form inactive dimers in a ligand-independent way through interactions between cytoplasmic domains. To date, the conformation of EGFR extracellular domain engaged in these inactive dimers remains unclear. In this study, we describe the successful selection and characterization of llama anti-EGFR nanobodies and their use as innovative conformational sensors. We isolated three different specific anti-EGFR clones binding to three distinct epitopes. Interestingly, the binding of all three nanobodies was found highly sensitive to ligand stimulation. Two nanobodies, D10 and E10, can only bind the ligand-free EGFR conformation characterized by an intramolecular tether between domains II and IV, whereas nanobody G10 binds both ligand-free and ligand activated EGFR, with an 8-fold higher affinity for the extended conformation in the presence of ligand. Here we took advantage of these conformational probes to reveal the existence of tethered EGFR in EGFR/ErbB2 predimers. These biosensors represent important tools allowing the determination of EGFR conformations and should help the design of relevant inhibitors. |
| Databáze: | OpenAIRE |
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