The Global Phosphoproteome of Chlamydomonas reinhardtii Reveals Complex Organellar Phosphorylation in the Flagella and Thylakoid Membrane
| Autor: | Matthew Juergens, Hongxia Wang, Leslie M. Hicks, Brian Gau, Ping Li, William O. Slade |
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| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Polymers Chlamydomonas reinhardtii macromolecular substances Biology Flagellum Thylakoids Biochemistry Analytical Chemistry Tandem Mass Spectrometry Phosphorylation Molecular Biology Titanium Research Algal Proteins Protein level Chromatography liquid Phosphoproteins biology.organism_classification Cell biology Flagella Thylakoid Proteome Chromatography Liquid |
| Zdroj: | Molecular & Cellular Proteomics. 13:2337-2353 |
| ISSN: | 1535-9476 |
| Popis: | Chlamydomonas reinhardtii is the most intensively-studied and well-developed model for investigation of a wide-range of microalgal processes ranging from basic development through understanding triacylglycerol production. Although proteomic technologies permit interrogation of these processes at the protein level and efforts to date indicate phosphorylation-based regulation of proteins in C. reinhardtii is essential for its underlying biology, characterization of the C. reinhardtii phosphoproteome has been limited. Herein, we report the richest exploration of the C. reinhardtii proteome to date. Complementary enrichment strategies were used to detect 4588 phosphoproteins distributed among every cellular component in C. reinhardtii. Additionally, we report 18,160 unique phosphopeptides at |
| Databáze: | OpenAIRE |
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