Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues
| Autor: | Yu Ri Choi, Guk Young Ahn, Ju Ri Park, Sung-Wook Choi, Min Jeong Lee, Tae-Kyung Ryu |
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| Rok vydání: | 2018 |
| Předmět: |
lcsh:Medical technology
Materials science Composite number Biomedical Engineering Medicine (miscellaneous) Composite 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Tissue engineering Biodegradable polymer Ultimate tensile strength chemistry.chemical_classification Universal testing machine Electrospinning technology industry and agriculture Polymer musculoskeletal system equipment and supplies 021001 nanoscience & nanotechnology Guided tissue engineering 0104 chemical sciences lcsh:R855-855.5 chemistry Chemical engineering Ceramics and Composites 0210 nano-technology Caprolactone Research Article Nanodiamond |
| Zdroj: | Biomaterials Research Biomaterials Research, Vol 22, Iss 1, Pp 1-8 (2018) |
| ISSN: | 2055-7124 |
| Popis: | Background Electrospun fibrous matrices are of great importance for tissue engineering and drug delivery device. However, relatively low mechanical strength of the fibrous matrix is one of the major disadvantages. NDs with a positive charge were selected to enhance the mechanical property of a composited fibrous matrix by inducing the intermolecular interaction between NDs and polymer chain. We prepared ND-composited poly (ε-caprolactone) (PCL) fibrous matrices by electrospinning and evaluated their performance in terms of mechanical strength and cell behaviors. Methods A predetermined amounts of NDs (0.5, 1, 2 and 3 wt%) were added into PCL solution in a mixture of chloroform and 2,2,2-trifluoroethanol (8:2). ND-composited PCL (ND/PCL) fibrous matrices were prepared by electrospinning method. The tensile properties of the ND/PCL fibrous matrices were analyzed by using a universal testing machine. Mouse calvaria-derived preosteoblast (MC3T3-E1) was used for cell proliferation, alkaline phosphatase (ALP) assay, and Alizarin Red S staining. Results The diameters of the fibrous matrices were adjusted to approximately 1.8 μm by changing process variables. The intermolecular interaction between NDs and PCL polymers resulted in the increased tensile strength and the favorable interfacial adhesion in the ND/PCL fibrous matrices. The ND/PCL fibrous matrix with 1 wt% of ND had the highest tensile strength among the samples and also improved proliferation and differentiation of MC3T3-E1 cells. Conclusions Compared to the other samples, the ND/PCL fibrous matrix with 1 wt% of ND concentration exhibited superior performances for MC3T3 cells. The ND/PCL fibrous matrix can be potentially used for bone and dental tissue engineering. |
| Databáze: | OpenAIRE |
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