Protective Effect of Indomethacin-loaded Polymeric Nanoparticles Against Oxidative Stress-Induced Cytotoxicity in Human Breast Adenocarcinoma Cell Model
| Autor: | Camila Franco, Altevir Rossato Viana, Aline Ferreira Ourique, Bruno Stefanello Vizzotto, Luciana Maria Fontanari Krause |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Revista Brasileira de Cancerologia; Vol. 68 No. 4 (2022): Oct./Nov./Dec.; e-012545 Revista Brasileira de Cancerologia; Vol. 68 Núm. 4 (2022): oct./nov./dic.; e-012545 Revista Brasileira de Cancerologia; v. 68 n. 4 (2022): out./nov./dez.; e-012545 Revista Brasileira de Cancerologia Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) instacron:INCA |
| ISSN: | 2176-9745 |
| Popis: | Introduction: Anti-inflammatory drugs are being utilized to treat cancer because of its inflammatory microenvironment. Objective: The objective of this study is to investigate the antioxidant potential of indomethacin and its genotoxicity, since free or loaded in polymeric nanocapsules using MCF-7 (human breast cancer) cells as an in vitro model. Method: Development of indomethacin-loaded polyepsiloncaprolactone (PCL) nanocapsules by interfacial deposition method. It is characterized by pH determination by potentiometer, mean diameter and polydispersity index by dynamic light scattering; zeta potential by electrophoretic mobility; encapsulation efficacy by high performance liquid chromatography method; corona effect formation; 2ʹ,7ʹ-dichlorofluorescin diacetate (DCFH-DA) method by spectrofluorimetric assay; nitric oxide (NO) determination by spectrophotometric and genotoxicity assay by plasmid DNA cleavage method. Results: The results showed a mild acidic pH (4.78 ± 0.10), sizes around 200 nm and PDI |
| Databáze: | OpenAIRE |
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