Immune response profile of caruncular and trophoblast cell lines infected by high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates of Neospora caninum
| Autor: | Esther Collantes-Fernández, Javier Regidor-Cerrillo, Nina Hambruch, Mercedes Gómez-Bautista, Christiane Pfarrer, Laura Jiménez-Pelayo, Pilar Horcajo, Marta García-Sánchez, Luis Miguel Ortega-Mora |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Placenta 030231 tropical medicine Cell Neospora caninum Biology Isolates Real-Time Polymerase Chain Reaction lcsh:Infectious and parasitic diseases Cell Line Andrology 03 medical and health sciences 0302 clinical medicine Immune system Pregnancy medicine Animals lcsh:RC109-216 Immune response Virulence Cell adhesion molecule Research Trophoblast Neospora biology.organism_classification Trophoblasts Caruncle 030104 developmental biology Infectious Diseases medicine.anatomical_structure Gene Expression Regulation Cell culture Cytokines Parasitology Tumor necrosis factor alpha Cattle Female |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 12, Iss 1, Pp 1-11 (2019) |
| ISSN: | 1756-3305 |
| Popis: | Background Bovine neosporosis, one of the main causes of reproductive failure in cattle worldwide, poses a challenge for the immune system of pregnant cows. Changes in the Th-1/Th-2 balance in the placenta during gestation have been associated with abortion. Cotyledon and caruncle cell layers form the maternal-foetal interface in the placenta and are able to recognize and induce immune responses against Neospora caninum among other pathogens. The objective of the present work was to elucidate the immunomodulation produced by high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates of N. caninum in bovine trophoblast (F3) and caruncular cells (BCEC-1) at early and late points after infection. Variations in the mRNA expression levels of toll-like receptor-2 (TLR-2), Th1 and Th2 cytokines (IL-4, IL-10, IL-8, IL-6, IL-12p40, IL-17, IFN-γ, TGF-β1, TNF-α), and endothelial adhesion molecules (ICAM-1 and VCAM-1) were investigated by RT-qPCR, and protein variations in culture supernatants were investigated by ELISA. Results A similar pattern of modulation was found in both cell lines. The most upregulated cytokines in infected cells were pro-inflammatory TNF-α (P |
| Databáze: | OpenAIRE |
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