Regulation of colony morphology and biofilm formation in Shewanella algae
| Autor: | Francisco Vilaplana, Ute Römling, Secil Yilmaz-Turan, Alberto J. Martín-Rodríguez, Åsa Sjöling, Katia Villion |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Shewanella
Virulence Bioengineering Shewanella algae Applied Microbiology and Biotechnology Biochemistry Microbiology 03 medical and health sciences Extracellular polymeric substance Algae Humans Research Articles 030304 developmental biology 0303 health sciences biology 030306 microbiology Transposon integration Chemistry Biofilm biology.organism_classification Mutagenesis Biofilms Transposon mutagenesis TP248.13-248.65 Bacteria Research Article Biotechnology |
| Zdroj: | Microbial Biotechnology, Vol 14, Iss 3, Pp 1183-1200 (2021) Microbial Biotechnology |
| ISSN: | 1751-7915 |
| DOI: | 10.1111/1751-7915.13788 |
| Popis: | Summary Bacterial colony morphology can reflect different physiological stages such as virulence or biofilm formation. In this work we used transposon mutagenesis to identify genes that alter colony morphology and cause differential Congo Red (CR) and Brilliant Blue G (BBG) binding in Shewanella algae, a marine indigenous bacterium and occasional human pathogen. Microscopic analysis of colonies formed by the wild‐type strain S. algae CECT 5071 and three transposon integration mutants representing the diversity of colony morphotypes showed production of biofilm extracellular polymeric substances (EPS) and distinctive morphological alterations. Electrophoretic and chemical analyses of extracted EPS showed differential patterns between strains, although the targets of CR and BBG binding remain to be identified. Galactose and galactosamine were the preponderant sugars in the colony biofilm EPS of S. algae. Surface‐associated biofilm formation of transposon integration mutants was not directly correlated with a distinct colony morphotype. The hybrid sensor histidine kinase BarA abrogated surface‐associated biofilm formation. Ectopic expression of the kinase and mutants in the phosphorelay cascade partially recovered biofilm formation. Altogether, this work provides the basic analysis to subsequently address the complex and intertwined networks regulating colony morphology and biofilm formation in this poorly understood species. In this work we observed a distinct phenotype of colonies formed by the marine indigenous bacterium Shewanella algae when grown on agar plates supplemented with dyes, and we used transposon mutagenesis to identify genes contributing to colony morphotype development and biofilm formation. Microscopic inspection of the colonies revealed distinct cell morphologies, arrangements, and extracellular matrix production in the wild‐type strain S. algae CECT 5071 and key transposon integration mutants representing the diversity of morphotypes. Electrophoretic and chemical analyses of extracted biofilm extracelluar matrix components of wild‐type and mutants showed differential fingerprints between strains, with galactose and galactosamine being the preponderant monosaccharides. Our work provides a basic analysis that paves the ground towards the dissection of the complex and intertwined networks regulating colony morphology and biofilm formation in this poorly understood species. |
| Databáze: | OpenAIRE |
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