In Vitro Production of Biotrophic-Like Cultures of Crinipellis perniciosa, the Causal Agent of Witches’ Broom Disease of Theobroma cacao
| Autor: | Ricardo Antunes Azevedo, Cláudia de M. Bellato, Johana Rincones, Lyndel W. Meinhardt, Gonçalo A.G. Pereira, J.C.M. Cascardo |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cacao
Mycelium biology Theobroma Broom fungi Cell Culture Techniques Moniliophthora General Medicine Fungus Spores Fungal biology.organism_classification Applied Microbiology and Biotechnology Microbiology Moniliophthora perniciosa Culture Media Spore Crinipellis Callus Botany Agaricales Plant Diseases |
| Zdroj: | Current Microbiology. 52:191-196 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-005-0182-z |
| Popis: | Witches' broom disease (WBD) of cacao, caused by the hemibiotrophic fungus, Crinipellis perniciosa, exhibits a succession of symptoms that are caused by the biotrophic phase of the fungus. However, the study of this biotrophic phase is limited by its exclusive growth inside the plant or in the presence of callus. Here we report for the first time a method for the growth and maintenance of the biotrophic-like phase of C. perniciosa on a defined medium with metabolites found in the diseased tissues. Our results suggest that glycerol is a key carbon source for this interaction. This is a crucial achievement toward understanding the biology of this fungus during the infectious phase of WBD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink