Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis
| Autor: | Lauralynn Kourtz, Kevin Dillon, Sean Daughtry, Oliver P. Peoples, Kristi D. Snell |
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| Rok vydání: | 2007 |
| Předmět: |
Polyesters
Transgene Arabidopsis Hydroxybutyrates macromolecular substances Polyhydroxybutyrate chemistry.chemical_compound Biosynthesis Dry weight Gene Expression Regulation Plant Genetics Gene Inducible gene biology technology industry and agriculture food and beverages Plants Genetically Modified biology.organism_classification Biosynthetic Pathways chemistry Biochemistry lipids (amino acids peptides and proteins) Animal Science and Zoology Agronomy and Crop Science Ecdysone Biotechnology |
| Zdroj: | Transgenic Research. 16:759-769 |
| ISSN: | 1573-9368 0962-8819 |
| DOI: | 10.1007/s11248-007-9067-1 |
| Popis: | Arabidopsis plants were transformed with a multi-gene construct for expression of the polyhydroxybutyrate (PHB) biosynthetic pathway containing a gene switch that can be activated by commercially available non-steroidal ecdysone analogs approved for use on some crops as pesticides. T(1) progeny of transgenic Arabidopsis plants were isolated and screened for PHB production in the presence of ecdysone analogs. T(2) progeny derived from selected T(1) lines were subjected to further analysis by comparing PHB production levels prior to treatment with inducing agent and 21 days after initiation of induction. Significant PHB production was delayed in many of the engineered plants until after induction. PHB levels of up to 14.3% PHB per unit dry weight were observed in young leaves harvested from engineered T(2) plants after applications of the commercial ecdysone analog Mimic. PHB in older leaves reached levels of up to 7% PHB per unit dry weight. This study represents a first step towards engineering a chemically inducible gene switch for PHB production in plants using inducing agents that are approved for field use. |
| Databáze: | OpenAIRE |
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