Hyalinizing clear cell carcinoma of salivary glands: A retrospective study focused on uncommon morphology, immunohistochemistry, and detection of gene fusion using fluorescence in situ hybridization
Autor: | Xiao Wang, Xiang-tao Song, Yi-nuo Zhao, Fen-hua Liang, Wen-jie Zhang |
---|---|
Rok vydání: | 2017 |
Předmět: |
Adult
Male Pathology medicine.medical_specialty Oncogene Proteins Fusion Calponin Perineural invasion Salivary Glands Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Stroma Eosinophilic medicine Biomarkers Tumor Humans 030223 otorhinolaryngology Hyalinizing clear cell carcinoma In Situ Hybridization Fluorescence Aged Retrospective Studies Gene Rearrangement biology medicine.diagnostic_test Cell Biology Middle Aged medicine.disease Salivary Gland Neoplasms Immunohistochemistry Staining 030220 oncology & carcinogenesis biology.protein Female Gene Fusion Fluorescence in situ hybridization Adenocarcinoma Clear Cell |
Zdroj: | Pathology, research and practice. 214(3) |
ISSN: | 1618-0631 |
Popis: | Aims To investigate histological, immunohistochemical, and molecular features, especially uncommon morphology of hyalinizing clear cell carcinoma (HCCC) to expand the morphological spectrum of HCCC. Methods and results We examined 5 cases of HCCC by histological, immunohistochemical, and molecular analysis. Generally, 5 HCCC cases shared similar characteristics, exhibiting clear to slightly eosinophilic cells arranged in cords, nests, islands, or trabeculae with a hyalinized stroma, while myxoid stroma, perineural invasion, and polygonal cells with high-grade nuclei were observed in 3 cases. Immunohistochemically, 5 cases were entirely immunoreactive for CKpan, whereas 80% HCCC cases were positive for P63, and CK14. None expressed immunoreactivity for S-100, Calponin, or GFAP. The positive rate of Ki-67 staining was about 5% in the classic area of case 3, but 40% in the high-grade area. As for the result of FISH findings, EWSR1 gene break was detected in all 5 HCCC cases. Conclusions Our study has expanded the morphological spectrum of HCCC, and proposed the diagnosis of HCCC should be confirmed by fully analyzing histological, immunohistochemical, and molecular features practically. |
Databáze: | OpenAIRE |
Externí odkaz: |