Polyphosphate:AMP Phosphotransferase and Polyphosphate:ADP Phosphotransferase Activities of Pseudomonas aeruginosa
| Autor: | Toshitada Noguchi, Kazuya Ishige |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Phosphotransferases (Phosphate Group Acceptor)
Base Sequence biology Chemistry Pseudomonas aeruginosa Polyphosphate Blotting Western Biophysics Adenylate kinase Cell Biology medicine.disease_cause biology.organism_classification Biochemistry Molecular biology Nucleoside-diphosphate kinase Phosphotransferase Polyphosphate kinase chemistry.chemical_compound medicine Molecular Biology Nucleoside Bacteria DNA Primers |
| Zdroj: | Biochemical and Biophysical Research Communications. 281:821-826 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.4415 |
| Popis: | In Pseudomonas aeruginosa PAO1, we have found massive polyphosphate:AMP phosphotransferase activity and polyphosphate:ADP phosphotransferase activity known as the reverse catalytic activity of polyphosphate kinase which participates in polyphosphate synthesis in the bacterium. Biochemical analysis using the partially purified polyphosphate:ADP phosphotransferase has revealed that it is independent of polyphosphate kinase and can function as polyphosphate-dependent nucleoside diphosphate kinase which most prefers GDP to the other three nucleoside diphosphates as a phospho-acceptor. It has been also demonstrated that polyphosphate:AMP phosphotransferase activity marked in the bacterium mainly originates from the combined action of the polyphosphate:ADP phosphotransferase described above and adenylate kinase. Both of the polyphosphate-utilizing activities require short polyP as a phospho-donor whose chain length is |
| Databáze: | OpenAIRE |
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