Immunogenicity of Decidual Stromal Cells in an Epidermolysis Bullosa Patient and in Allogeneic Hematopoietic Stem Cell Transplantation Patients
| Autor: | Pia Molldén, Xiaoguang Li, Silvia Nava, Tom Erkers, Helen Kaipe, Takashi Hashimoto, Behnam Sadeghi, Hua Qian, Mats Alheim, Britt Gustafsson, Olle Ringdén, Lena-Maria Carlson |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Stromal cell Adolescent medicine.medical_treatment Graft vs Host Disease Hematopoietic stem cell transplantation Mesenchymal Stem Cell Transplantation Peripheral blood mononuclear cell Mice Original Research Reports HLA Antigens Decidua medicine Animals Humans Immunogenetic Phenomena Child Aged integumentary system biology Mesenchymal stem cell Hematopoietic Stem Cell Transplantation Infant Mesenchymal Stem Cells Serum Albumin Bovine Cell Biology Hematology Middle Aged medicine.disease Antibodies Anti-Idiotypic Immunology Leukocytes Mononuclear biology.protein Heterografts Female Epidermolysis bullosa Antibody Epidermolysis Bullosa Cell Adhesion Molecules Junctional epidermolysis bullosa (veterinary medicine) Developmental Biology Hemorrhagic cystitis |
| Zdroj: | Stem Cells and Development. 24:1471-1482 |
| ISSN: | 1557-8534 1547-3287 |
| Popis: | Allogeneic mesenchymal stromal cells (MSCs) are widely used in regenerative medicine, but little is known about their immunogenicity. In this study, we monitored the therapeutic and immunogenic effects of decidual stromal cells (DSCs) from term placentas when used as a therapy for generalized severe junctional epidermolysis bullosa (JEB) (previously termed Herlitz JEB), a lethal condition caused by the lack of functional laminin-332. An 11-month-old JEB patient was treated with five infusions of allogeneic DSCs within a 3-month period. Amniotic membranes (AMs) were applied to severe wounds. After the treatment, wounds started to heal in the middle of the blisters, but the improvements were transient. After two infusions of DSCs, the JEB patient had developed multispecific anti-HLA class-I antibodies. No antibodies to laminin-332 were detected, but the patient had high levels of anti-bovine serum albumin antibodies, which could bind to DSCs. Peripheral blood mononuclear cells (PBMCs) from the patient had a higher proliferative response to DSCs than to third-party PBMCs, which contrasts with the pattern observed in healthy donors. Human DSCs and MSCs induced similar xenoreactivity in mice. Two of 16 allogeneic stem cell-transplanted patients, treated with DSCs for graft-versus-host disease or hemorrhagic cystitis, showed a positive flow cytometric crossmatch test. One patient had anti-HLA antibodies before DSC infusion, whereas the other had no anti-HLA antibodies at any time. AM and DSC infusions may have improved the healing process in the JEB patient, but DSCs appeared to induce anti-HLA antibodies. The risk of alloimmunization by DSCs seems to be low in immunocompromised patients. |
| Databáze: | OpenAIRE |
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