Engraftment of HTLV-I-Transformed Human T-Cell Line into SCID Mice with NK Cell Function
| Autor: | T. Ohsugi, M. Shingu, K. Ishibashi, T. Nomura |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.drug_class T-Lymphocytes T cell Molecular Sequence Data Transplantation Heterologous Pcr assay Fluorescent Antibody Technique Mice SCID Biology Scid mice Kidney Immunofluorescence Monoclonal antibody Polymerase Chain Reaction Mice chemistry.chemical_compound medicine Animals Humans Leukemia-Lymphoma Adult T-Cell Lung Gene Cell Line Transformed DNA Primers Human T-lymphotropic virus 1 Base Sequence General Veterinary medicine.diagnostic_test Cell function Molecular biology Killer Cells Natural Cell Transformation Neoplastic medicine.anatomical_structure Liver chemistry Lymphocyte Transfusion DNA Viral Female Lymph Nodes Spleen DNA |
| Zdroj: | Journal of Veterinary Medical Science. 56:601-603 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.56.601 |
| Popis: | HTLV-I-transformed human T-cell line, MT-2, has been reported to successfully proliferate in only the SCID mice with depletion of NK cell function. However, MT-2 cells could be engrafted into SCID mice possessing normal NK cell function in this study. MT-2 cells (5-7 x 10(7) cells) were intraperitoneally injected into SCID mice without the deletion of NK cell function. The SCID mice developed tumors in the peritoneal cavities 3 months after the inoculation of MT-2 cells. All tumors reacted to anti-HTLV-I p19 and anti-HLA-DR monoclonal antibodies by immunofluorescence assay and were also positive for the HTLV-I gene by PCR assay. DNA obtained from main organs in group of mice with or without tumors showed a high incidence of positive signals for HTLV-I gene by PCR assay. |
| Databáze: | OpenAIRE |
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