Adenovirus-mediated gene transfer of basic fibroblast growth factor promotes the survival of primary-cultured rat neuronal cells
| Autor: | Masayuki Akimoto, Hisae Mori, Shin-Ichi Miyatake, Norihiro Matsuoka, Jun Takahashi, Hirofumi Hamada, Masaaki Saiki, Kazuhiro Ishii, Hiroyuki Yukawa, Nobuo Hashimoto |
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| Rok vydání: | 2000 |
| Předmět: |
Interleukin 2
Cell Survival Adenoviridae Infections medicine.medical_treatment Genetic enhancement Basic fibroblast growth factor bcl-X Protein Enzyme-Linked Immunosorbent Assay Biology Adenoviridae Viral vector chemistry.chemical_compound medicine Animals Humans Vector (molecular biology) Rats Wistar Cells Cultured Neurons General Neuroscience Growth factor Gene Transfer Techniques In vitro Rats Cell biology Proto-Oncogene Proteins c-bcl-2 chemistry Cell culture Immunology Interleukin-2 Fibroblast Growth Factor 2 medicine.drug |
| Zdroj: | NeuroReport. 11:2001-2006 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-200006260-00039 |
| Popis: | We constructed two replication-deficient recombinant adenovirus vectors coding human basic fibroblast growth factor (bFGF), one with and one without the interleukin-2 (IL-2) secretory signal sequence and examined their neurotrophic effects on primary neuronal cells in vitro. The primary neuronal cells were successfully infected at a high efficiency with the adenovirus vectors. bFGF protein was detected in the culture medium of the neurons infected with both these vectors. The cells infected with the bFGF-expressing adenovirus containing the IL-2 signal sequence showed 2- to 10-fold higher levels of secretion levels than cells infected with the native bFGF-expressing adenovirus alone. Both bFGF-expressing vectors augmented the survival of primary neuronal cells in an in vitro culture, compared with a mock infection or control virus infection. Notably, the cells infected with the bFGF-expressing adenovirus containing the IL-2 signal sequence were markedly enhanced cell survival in the early phase of the culture, compared with the control cells and even those infected with the bFGF-expressing adenovirus without the IL-2 signal sequence. However, in the late phase of neuronal culture, neither viral vector could support the cell survival. In contrast the co-infection of the bFGF-expressing vector with a Bcl-xL-expressing vector was extremely effective on neuronal survival. |
| Databáze: | OpenAIRE |
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