Ciprofloxacin enhances stress erythropoiesis in spleen and increases survival after whole-body irradiation combined with skin-wound trauma

Autor: Juliann G. Kiang, True M. Burns, Risaku Fukumoto
Rok vydání: 2013
Předmět:
Mouse
lcsh:Medicine
Bone Morphogenetic Protein 4
Mice
Ciprofloxacin
Erythropoiesis
lcsh:Science
Skin
Kidney
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Anemia
Animal Models
Hematology
Haematopoiesis
Radiation Injuries
Experimental
medicine.anatomical_structure
Medicine
Female
Aplastic Anemia
Radiology
Whole-Body Irradiation
Veterinary Pathology
medicine.drug
Research Article
medicine.medical_specialty
Histology
Immunoblotting
Immunology
Spleen
Immunopathology
Immunomodulation
Model Organisms
Stress
Physiological
Internal medicine
Veterinary Pharmacology
medicine
Splenocyte
Animals
Immunologic Factors
Erythropoietin
Biology
Wound Healing
business.industry
lcsh:R
Radiobiology
medicine.disease
Bone Marrow Failure
Survival Analysis
Hematopoiesis
Endocrinology
lcsh:Q
Veterinary Science
business
Wound healing
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 2, p e90448 (2014)
ISSN: 1932-6203
Popis: Severe hematopoietic loss is one of the major therapeutic targets after radiation-combined injury (CI), a kind of injury resulting from radiation exposure combined with other traumas. In this study, we tested the use of ciprofloxacin (CIP) as a treatment, because of recently reported immunomodulatory effects against CI that may improve hematopoiesis. The CIP regimen was a daily, oral dose for 3 weeks, with the first dose 2 h after CI. CIP treatment improved 30-day survival in mice at 80% compared to 35% for untreated controls. Study of early changes in hematological parameters identified CI-induced progressive anemia by 10 days that CIP significantly ameliorated. CI induced erythropoietin (EPO) mRNA in kidney and protein in kidney and serum; CIP stimulated EPO mRNA expression. In spleens of CI mice, CIP induced bone morphogenetic protein 4 (BMP4) in macrophages with EPO receptors. Splenocytes from CIP-treated CI mice formed CD71⁺ colony-forming unit-erythroid significantly better than those from controls. Thus, CIP-mediated BMP4-dependent stress erythropoiesis may play a role in improving survival after CI.
Databáze: OpenAIRE
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