Determination of procaine in equine plasma and urine by high-performance liquid chromatography
| Autor: | A. J. Stevenson, M. P. Weber, F. Todi, M. Mendonca, J. D. Fenwick, L. Young, E. Kwong, F. Chen, P. Beaumier, S. Timmings, D. Woodard, S. Kacew |
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| Rok vydání: | 1992 |
| Předmět: |
Detection limit
Doping in Sports Chemical Health and Safety Chromatography Penicillin G Procaine Chemistry Health Toxicology and Mutagenesis Urinary system Procaine Hydrochloride Urine Toxicology High-performance liquid chromatography Analytical Chemistry Procaine Blood plasma medicine Environmental Chemistry Animals Horses Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of analytical toxicology. 16(2) |
| ISSN: | 0146-4760 |
| Popis: | The variability in plasma and urine equine procaine measurement between three independent laboratories using current methods led to the development of a sensitive, reliable, and reproducible high-performance liquid chromatographic method. Standardbred mares were administered either a penicillin G procaine preparation intramuscularly or procaine hydrochloride subcutaneously, and blood and urine were collected at defined time intervals. By HPLC the detection limits for procaine in plasma and urine were 1 and 10 ng/mL, respectively. In contrast procaine in plasma could not be detected by GC-NPD, while the urinary detection limit was 50 ng/mL. The concentration of fluoride in the collection tubes and repetitive freeze-thawing modified plasma procaine measurement. Urinary pH was a factor in estimation of urine procaine levels with greater recovery and reproducibility of results at pH 5 as compared to pH 7. This HPLC method provides a simple, sensitive, and reliable quantitation of procaine in equine plasma and urine. |
| Databáze: | OpenAIRE |
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