Altered cofactor regulation with disease-associated p97/VCP mutations

Autor: Conrad C. Weihl, You Jin Lee, Pei Yin Shih, Valentina Sanghez, Henry J. Lin, Michelle R. Arkin, Lin Gui, Daniel E. Wong, Michelina Iacovino, Tsui-Fen Chou, Stacie L. Bulfer, Xiaoyi Zhang, Xiaoyan Zhang, Yanzhuang Wang, James Siho Lee, Lynn W. Lehmann
Rok vydání: 2015
Předmět:
Zdroj: Proceedings of the National Academy of Sciences of the United States of America, vol 112, iss 14
ISSN: 1091-6490
Popis: Dominant mutations in p97/VCP (valosin-containing protein) cause a rare multisystem degenerative disease with varied phenotypes that include inclusion body myopathy, Paget’s disease of bone, frontotemporal dementia, and amyotrophic lateral sclerosis. p97 disease mutants have altered N-domain conformations, elevated ATPase activity, and altered cofactor association. We have now discovered a previously unidentified disease-relevant functional property of p97 by identifying how the cofactors p37 and p47 regulate p97 ATPase activity. We define p37 as, to our knowledge, the first known p97-activating cofactor, which enhances the catalytic efficiency (k_(cat)/K_m) of p97 by 11-fold. Whereas both p37 and p47 decrease the K_m of ATP in p97, p37 increases the k_(cat) of p97. In contrast, regulation by p47 is biphasic, with decreased k_(cat) at low levels but increased k_(cat) at higher levels. By deleting a region of p47 that lacks homology to p37 (amino acids 69–92), we changed p47 from an inhibitory cofactor to an activating cofactor, similar to p37. Our data suggest that cofactors regulate p97 ATPase activity by binding to the N domain. Induced conformation changes affect ADP/ATP binding at the D1 domain, which in turn controls ATPase cycling. Most importantly, we found that the D2 domain of disease mutants failed to be activated by p37 or p47. Our results show that cofactors play a critical role in controlling p97 ATPase activity, and suggest that lack of cofactor-regulated communication may contribute to p97-associated disease pathogenesis.
Databáze: OpenAIRE